Week of 4/29/24-5/3/24 Plasmid Extraction Continued

 We made Urease solution for several plates, 2 plates were inoculated with Staph and Subtillus. This was also a facet through which we could observe mechanotaxis and chemotaxis motility phenomena. On Tuesday we inoculated two plates of R2A with Sonorensis in order to redo plasmid extraction. On Wednesday, we performed the plasmid extraction finally, using eight plates and increase the radiation by 25,000 mj/cm squared per plate. One plate was our control, which was not exposed to any radiation. We also needed to fix our dilution factor for non-iradiated bacteria, it was too high compared to what we established for our experiment. On Friday we listened to a presentation about epigenetics and methylation. Methylation occurs near regions with hemimethylation. We also observed hypomethylation. The process for epigenetics includes starting with initial growth of bacteria, then performed DNA isolation. From there, we do DNA fragmentation, and continue forth with Bisulfide conversion. With which nanopore sequencing, then we find epigenetic marks with which to compare sequences to find methylation. We talked about the use of Dam2DR, DNA reuptake, and gene expressing at 6MA amount. 

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