Week of 8/26/24 Nanodrop Values and Gel

 The values the nanodrop had give for pgRNA were of the following: 78.0 (ng/ul),  1.87 (A260/A280), 2.22 (A260/A230). PWTCas9 58.4 (ng/ul), 1.85 (A260/A280), 2.09 (A260/A230). Prahm- 46.1 (ng/ul), 1.92 (A260/A280), and 2.09 (A260/A230). Performed plasmid extraction on Cas9  and gram stained plain Sonora. 250ml of LB broth were made as well as a gel. Several passages were also performed, on PWTCas9, D. sonora, and pgRNA. We had to reattempt PWT transformation as it could not be confirmed. This could be due to contamination, or not enough cells were present within the transformation protocol. Both a broth and a plate of R2A were inoculated with Sonora, we thought it would be best if we could successfully have transformed Sonora growing within a broth. 

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