Week of 9/9/24 Retransformation of D. sonora and verification

 We gram stained our ampicillin plates containing pwtcas9 D. sonora (0.2ug/ml). The gram stain was verified to be consistent with the shape of short rods and gram positive. Then, we passaged this onto another plate of TGY ampicillin plates to keep this culture going. In order move forward with ligation of the pwtcas9 with xbam and xbai restriction sites for the kanamycin resistance gene. From there, we loaded a gel to prepare for gel excision. In making our restriction enzyme xBai, we had come across a calculation error in which the concentration of xbai was too concentrated. We had calculated 2 1/2 times more than the 1 ratio we needed. 

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