Week of 10/14/24

 When we ran the gel on Pgk and PgRNA we found that both were about the size of 200bp, which means that they were not successful. So we did religate PgK. We did another gel just to confirm these results due to the fact the 1KB ladder was not viable. We also increased our insert to vector molar ratio to 5:1 to have a better chance of the insert coming together with the vector. 

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