Week of 10/21/24

 We redid PCR attempt due to a discrepancy of verification. We also did plasmid extraction in order to get more sample of Pdcas9. We are deciding if a new verification process to narrow down the issue with Xbai ligation using lambda would be viable. More chloremphenicol plates for passaging e.coli pdcas9 were made. 

Comments

Post a Comment

Popular posts from this blog

Week of 4/22/24-4/26/24 Remaking Media, Urease Plates, and Competent Cells

 Upon returning to lab, we observed that our previous media containing R2B had reached a timeout due to not being autoclaved properly. In the future, more emphasis will be accrued to ensuring media and respecting specimens are to be autoclaved. Therefore, we had to once again remake R2B media and TGY media. We curated three flasks of R2B with Deinococcus Sonorensis in order to perform plasmid extraction, if there was a plasmid to extract in the first place. We did this via the zippy kit. We also poured urease plates, and inoculated four flasks of R2B with Xinjiangensis to continue forward with our UV testing. Sonorensis was observed to have formed white plaque within R2B, possibly due to the TGY component therein. We then took 1ml each in eight epindorph tubes. Centrifuged that for 1 minute at 13,000 rpm. Thereafter discarding the super natent, ensuring OD values of 1, and repeating the process of centrifuging if needed to enlarge the bacterial pellet. We made it a point to inocula...
Read more

Week of 11/22/24 pH Experiment on Sonora's Biofilm Breakdown

Image
 After incubating the pH range of 6,7, and 8 the cells were gram stained after 24 hrs along with a plain TGY broth containing Sonora for a comparison and all of the flasks were homogenized strangely enough. The morphology was still irregular as if the cells were still dying. The gram stains albeit were not completely viable, as there was a variable gram negative to gram positive ratio. The OD values were taken as well, and the results were also varied as pictured below.
Read more