Week of 10/28/24

 Moved forward with single digestion of Kana fragment using Xbai. D. sonora plain was also passaged onto TGY and R2A. Our pdCas9 of Sonora was contaminated, so we have to redo transformation and possibly plasmid extraction. For single digestion of PgRNA it was digested and ligated with Xbai first then BAM digestion and ligation.

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