Week of 9/17/24

 We passaged PWT-Cas9 onto plates with TGY and varying amounts of Ampicillin concentrations. The concentration was 0.4 ug/ml on two plates, 0.6ug/ml on two plates, and 0.8ug/ml on two plates. This was the method in which we decided on verifying for successful transformation of D. sonora. However, upon doing this passage we discovered that using PWT-Cas9 would not be viable to use in conjunction with sonora due to its innate insanely high tetracycline resistance. We are deciding on moving forward using PdCas9 as we are able to use Chloremphenicol instead. 

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