Week of 3/18/24-3/22/24 Media Prep for Biochemical Tests

 Introduction

 Prior to performing biochemical tests on Xinjiangensis, E.coli, Epidermis, and Citroraptor we made SIM media, R2B, and TGY. The biochemical tests involved included indole, starch, sulfur, motility, and urease. I was in charge of the amylase test in order to determine if Xinjiangensis utilizes starch for energy. As for the purpose of the indole test it was prepared to determine if Xinjiangensis can split tryptophan into pyruvate and indole end products. The motility test was used to determine whether or not the bacterium can spread/move. The indole test's purpose was to determine if Xinjiangensis is coded for the tryptophen protein which is found in most proteins. Once more, the sulfur test in regards to Xinjiangensis was used to characterize whether or not the bacterium can reduce sulfur into hydrogen sulfide. Finally, the urease test was used to characterize if Xinjiangensis harbored the enzyme necessary to break down urease into ammonia and carbon dioxide. 


Methods

In order to perform the amylase test we needed to streak the bacterium (Xinjian., E.coli, Citro, Epidermis) onto several plates. From there, we put 5-10 drops of IKR around the area of each bacterium. In order to determine if Xinjiangensis utilizes starch as energy, the parameter of the bacterium would appear to have a separation between the IKR that turned black and the bacterium streaks themselves. This was easily discernable, as compared to that of Epidermis which garners a negative result. 


Results

Upon dispensing the IKR onto XInjiangensis, the bacterium does appear to utilize starch as a means of energy. The separation between the blackness of the sulfur around the parameter versus that of the discolored "wall" can be observed. 

Discussion

Unfortunately, the Xinjiangensis was contaminated, so we had to reinoculate the bacterium once again. This may have been due to the lack of an aseptic approach.  

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