Week of 4/15/24-4/19/23 Making Media and Adjusting Project Processes


We remade R2A and TGY broth to conduct another set of UV resistance tests on Xinjiangensis. This time, the revised protocol for such has been tweaked to contain a spectrum of UV radiation levels rather than merely a jump from 50 mj/cm squared and 100 mj/cm squared to include differing intensities of UV exposure as a spectrum such as 25 mj/cm squared, 50 mj/cm squared, 100 mj/cm squared, and values in between of such UV exposure. Another process we fixed was from the specific inoculations of each bacteria colony to only consist of one, as to eliminate a technical error to one biological rep. The recoverability of R2A and TGY respectively includes 6 flasks, one for each UV test labeled A, B, C. 25ml of TGY per tube. Each tube of broth was alloclotted to reduce contamination, diluted, inoculated onto 11 plates, and then exposed to UV values. The purpose for doing so is to replicate this process for more verifiable and conclusive results as opposed to our prior tests. We want to understand and know if the media the bacteria is grown in matters with respects to surviving varying levels of UV exposure. Initially we made two flasks, one was TGY and the other was R2A. From there, we attained an OD value of 1, once verified we pipetted 50 microliters of each onto parafilm. Then we diluted each in 450ml of TGY and R2A into epindorph tubes accordingly. We had 10 plates of R2A divided up into thirds of A, B and C which was then exposed to UV values. We had one control plate, which was exposed to 0k UV. Our revised process was concluded to contain just two flasks of R2B, attained an OD value of 1 each, pipette 50ml onto parafilm, exposed to differing levels of UV this time more so of a spectrum, and then took 20ml afterwards into 180ml of R2B. Then, we inoculated each onto a plate of R2B which was divided up into four quadrants. 





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