Week of 10/1/24

 We did PCR on the ligated Kanamycin fragment in order to replicate more of our sample to able to load it onto a gel for confirmation and future uses. We also transformed e.coli with the PCR product of the Kana fragment so that way we can perform plasmid extraction and transform sonora with the kana fragment as well. We also redid double digestion once more just to ensure that it is the Xbai that is the problem in conjunction with the BAM fragment. We did this via a 3:1 molar ratio of insert to vector as the New England protocol for double digestion calls for. 

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